Open the catalog to page 1. State-of-the-art Multi-Mode Microplate Reader. 00. The OD 600 was plotted against time using Graphpad Prism 8. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. Figure 2: Images of colonies of commonly used cell types raised in semi-solid culture in CloneMedia. The drawing shows a standard microplate of 15 mm height covered with a lid. ] Example of document to evaluate if the reader . The experiment was carried out with four biological replicates and two technical replicates. With optimized reagents, option to operate the. , Sunnyvale, CA, USA) and normalized to the absorbance in the. When recombinant supernatants were tested for the presence of Il10, only values within the linear range of. • H1_M_FI_T_MUB. URL of this page: HTML Link: Bookmark this page. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. The absorbance of the samples was determined using a FilterMax F3&F5 Multi-Mode Microplate Reader (Molecular Devices). Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . We recommend you read this assay in dual emission mode, detecting both the emission from the Europium donor fluorophore at 615 nm, and the acceptor fluorophore (at 665 nm for APC. All experiments were performed in triplicate. CloneMatrix. Enzymatic activity was calculated after subtracting absorbance decay obtained from buffer without cell lysates by using NADPH extinction coefficient of 6220/M/cm and by normalizing to total protein. 3 software (Molecular Devices). Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter incubation for 6 minutes, the mixture was read at 340 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Readers, Molecular Devices). F3 F5 F3-SC F5-SC F3 F5. Neurotransmitter Transporter Uptake Assay Kit. After 24 h, the. , Sunnyvale, CA, USA). 8 Pages. 2 Pages. MetaMorph NX. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Coculture experiment. 2. 50/50. SpectraMax Glo Steady-Luc Reporter Assay Kit. MDA is one of the final products of LPO. shopping cart 0 items $0. A 1:1 binding ratio Hill model was used to fit the binding interaction data. Catalog. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. A photomultiplier tube (PMT) converts a light pulse into an electrical signal of measurable magnitude. 030 fmol Fluorescence Polarization (FP). performs within its specifications. Cell viability was measured in percentages (%) as compared to untreated cells, which were assumed to be 100% viable. 380 or. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. Lonza Luminometers Instrument MycoAlert™ Assay MycoAlert™ PLUS Assay Other Bioassays* Notes Lonza Lucetta™ Luminometer + + Cuvette Tailor -made MycoAlert™ Mode Luminometers from Other SuppliersTriplicate reactions were measured every 2. 7°C and the bottom to 36. Equal concentrations of protein were separated in Mini-PROTEAN® TGX™ Precast 4–20% Gels (Bio-Rad, Hercules, CA, USA) and transferred to nitrocellulose. 01% in 2030. SpectraMax Glo Steady-Luc Reporter Assay Kit. x. Vascular. FilterMax F5 Multi-Mode Microplate Reader. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Dave Zeckhausen. The samples were transferred to black 96-well plates and fluorescence was measured at an excitation wavelength of 405 nm and an emission wavelength of 535 nm on the FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices) equipped with the SoftMaxPro Microplate Data Acquisition and Analysis 6. The microplates were incubated at 37°C for 24 h, and the growth of bacteria was determined by measuring the optical density (OD) at 630 nm using an ELISA microplate reader (FilterMax F5 multi-mode microplate reader, Molecular Devices, Sunnyvale, CA, USA). 0´ 104 8. Z’-LYTE® Compatible Microplate Reader Documentation Version No. Each condition was performed in. Description Z-height focusing is enabled for fast top- and bottom-reads for all read modes. 375% (w/v) thiobarbituric acid was added. The medium was then removed and the cells washed once with 200 μl of ice-cold phosphate buffered saline (PBS). Download Table of Contents. Ease of use. SpectraMax Glo Steady-Luc Reporter Assay Kit. Conquer Scientific provides laboratories around the world high quality, low-cost pre-owned laboratory instruments. The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 97059-524EA 84727. At day 4 P. 00 SpectraMax M3, M4, M5, and M5e Multi-Mode Document type: User Manual5016208D 8 Chapter1:Introduction TheFilterMax™ F3Multi-ModeMicroplateReaderandtheFilterMax™F5Multi-Mode MicroplateReaderareidealforabroadrangeofapplications. Cells were co-transfected with 2 μg total plasmid DNA (1760 ng empty vector or proviral plasmid DNA. The reactions were analyzed in a fluorometer (F5 Multi-Mode Microplate Reader) at 590 nm after a 120-h incubation. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. F5 Multi-Mode Microplate Reader. shopping cart 0 items $0. They provide absorbance, fluorescence, glow. Learn how to set up protocols, customize reports, export results, and more with this PDF document. The papers, which were published between 2010 and. SpectraMax Paradigm Multi-Mode Microplate Reader. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. A powerful and reliable microplate reader is what you need! Get access to readers which will ensure an optimal readout. After adding 100 μl of new ice-cold PBS to the cells, fluorescent intensity was immediately determined using the fluorescent plate reader (FilterMax F3&F5 Multi-Mode Microplate Reader, Molecular Devices). At least three independent experiments were performed for each. 3 Pages. 3D cell proliferation assays were conducted using high concentrations to optimize conditions for cell lines in 20, 100 or• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. 40266 Email: drugdiscoverytech@lifetech. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. Protein quantification was carried out using the Pierce™ BCA Protein Assay Kit (Thermo Fisher) and a FilterMax F5 Multi-Mode Microplate Reader (San Jose, CA, United SA). Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. Transfluor Technology. Laser-aided direct metal deposition of metals and alloys. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. 2 Pages. The transmittance at 450 nm was determined by FilterMax F5 Multi-Mode Microplate Reader (Molecular devices, CA, USA). 5. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. Click on the microplate icon in the upper left corner and then click FilterMax™ F5 Multi-Mode Microplate Reader: Supplier No. The hemolysis was calculated with the following formula: Hemolysis (%) = (A sample − A PBS)/(A Triton − A PBS. The measurement can be performed using a variety of detection technologies: the. FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. The VMax® Kinetic ELISA Microplate Reader, the first of its kind for monitoring reactions over time, opened the door to enzyme assays and continues to be the workhorse of the laboratory environment. ScanLater Western Blot Detection System. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. 3 Pages. Luminescence (glow) Fluorescence polarization. Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader + + Molecular Devices FlexStation. Trinean Dropsense 96 Polychromatic. MetaFluor Software. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Activation of TLR4 and TLR2 receptors. 4 Pages. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $7,900. Category 2 and 3 microplate readers use gradient heating as well. MetaMorph NX. FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices Supplier: Molecular Devices. The next day, precultures were diluted to an optical density of 0. 5. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. The amount of each HTRA1 protein was. Instrument Configuration: Assay Technology Adapta® & Setup for GeneBLAzer® Assay on Molecular Devices FilterMax™ F5 Microplate Reader with SoftMax® Pro 6. Microplate formats can vary from 6-1536 wells, providing the ability to read many samples in a short time. SpectraMax Gemini XPS/EM Microplate Readers. CloneMatrix. Compare Fluorescence Microplate Readers from leading suppliers on Biocompare. Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader. Instrument Configuration: Assay Technology Adapta® & • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate. Absorbance values recorded at 490 nm using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices) were then analyzed with Excel software (Office, Microsoft) and GraphPad Prism 6 software (La Jolla, CA, USA) to assess statistical significance. . The 0. Seoul, South Korea. The MTT assay was performed with 100 μM vitamin E in the presence or absence of LPS at a concentration of 1 µg/mL for 24 h. Molecular Devices FilterMax F5 User Manual (49 pages) Multi-Mode Microplate Readers. Generally when this occurs, the destination BIG-IP device is unable to execute the tmsh command successfully. Create an experiment based on the protocol. Neurotransmitter Transporter Uptake Assay Kit. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. C1–F5 Filter Set: EX 360/40 nm EM 460/40 nm Optics Position: Gain: Auto Scale to High Wells Scale Well: C1 Scale Value: 30000 Delay after Plate Movement: 100 msec Synergy. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. The data were analyzed using Multi-Mode Analysis software and normalized to fluorescence in the vehicle-treated cells (% of control). Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). An anti-human HER2/neu human IgG3/κ (Huang and Morrison, 2006) was used as isotype (negative) control. Enzymatic activity was calculated after subtracting absorbance decay obtained from buffer without cell lysates by using NADPH extinction coefficient of 6220/M/cm and by. ” e. ” e. Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. Upgradeable modules are also available including western blot, cell imaging, and fast. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. Absorbance was measured at 450 nm using a FilterMAx F5 Multi-Mode Microplate Reader and EX2 filter after a 2-hour incubation at 37°C with 10 μL of CCK-8 per 100 μL of media. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535 nm, 595 nm, 625 nm; FP: 535 nm x 2), Excitation Filter Slide #2 (Absorbance: 260 nm, 340 nm, 405 nm, 450 nm, 595 nm, 620 nm), SoftMax Pro Microplate Data Acquisition and. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. , Sunnyvale, CA, USA). Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 2 Pages. Perception (Vial Rack Bottom Label 2D Reader) Microscan. The colorimetric signal development was stopped after 10 minutes. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 003 fmol 0. By category. Other technologies such as imaging. Z’-LYTE® Compatible Microplate Reader Documentation Version No. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataFilterMax ™ F5 Multi-Mode Microplate Reader; SpectraMax L Microplate Luminometer; Methods. 2 mm) was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 15 min over 16 h incubation time. Instrument Configuration: Assay Technology Adapta® & FilterMax F5 Multi-Mode Microplate Reader. 4 Pages. Page 154: Ordering Parts And Accessories. The Labsystems Nepheloskan Ascent Microplate Reader is designed to run with 96-well microplates, for use on applications like immunoprecipitation reactions as well as solubility tests of pharmaceutical components. FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. 00. Instrumentation and Quality Control. The amount of the colorimetric product was monitored continuously for 120 min. The fluorescence was determined using a fluorimetric microplate reader (FilterMax F5 Multi-Mode Microplate Reader) at 540 and 595 nm for excitation and emission, respectively. The values of luminescence are directly proportional to the number of viable cells. 40266 Email: drugdiscoverytech@lifetech. $3,000 USD. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The increase in NADH, an end product in this reaction, was determined by means of its light absorbance at 355 nm using a fluorometric microplate reader (FilterMax F5 Multi-Mode Microplate Reader; Molecular Devices,. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. Manufacturer: Molecular Devices. com A. Description: The FilterMax™ F3 features absorbance (visible),. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. SpectraMax iD5. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. , GFP expression yields from High Five cells infected with the two viruses were estimated using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Page 137 Using the same plate as was used in step 3, pipette 50 µL/well of buffer • into wells C1–F5 and C10–F12. Transfluor Technology. An MTT assay was performed with 5 mM BHB in the presence or absence of LPS (1 µg/mL) for 24 h. Designed for researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX Multimode Microplate Reader offers features that help save time and reduce common errors in the lab—including automated. The inter-assay coefficient of variations (CV) and values for all biomarkers can be found in Table 4. Installation Qualification (IQ) FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. Share. 100 fmol 384-well (75 µL) 0. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The instrument performs an initialization procedure that moves the optics and the microplate drawer to home positions. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Optical System: High energy xenon flash lamp. 5 mP. Close Search. 012 fmol 0. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). S. Page 24: Synergy™ Ht Multi-Mode Microplate Reader Internal plate shaking is also supported. , San Diego, CA, USA). FilterMax F3. Absorbance was measured at 485 nm with FilterMax F5 Multi-Mode microplate readers and the percentage of viable cells was calculated in relation to cells cultured in pure medium. Mettler Jr. 40266 Email: drugdiscoverytech@lifetech. Click on the microplate icon in the upper left corner and then click "New" to open a new data file: In the section "Plate1", click on the Settings icon. Time-resolved Fluorescence. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. Fluorescence polarization was measured at 21°C using FilterMax™ F5 Multimode Microplate Reader (Molecular Devices) with excitation and emission wavelengths of 485 nm and 535 nm, respectively. F5 has made an exception to this policy for BIG-IP 13. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. 4. 00. Select the protocol “Concentration_pico_green_v3. 7. After 30 min, the reaction was stopped by adding 1 N HCl and absorbance at a wavelength of 490 nm was measured using the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. CatchPoint cGMP Detection Assay Kit. Configurability is an important aspect of BioTek’s multi-mode readers to provide the most value for laboratory budgets, but the independent optical systems in our multi-mode readers don’t compromise on performance. Set up plate with cells, reagent and test compounds. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. S. SpectraMax® M5/M5e Microplate Reader & FlexStation® 3 Benchtop Multi-Mode Microplate ReaderSpectraMax M-series Microplate readers. 8. Microplate Readers. FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices SpectraMax 340PC384 Microplate Reader, Molecular Devices. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to. 3. The planktonic growth inhibition (%) was calculated according to the following equation:Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. Chemiluminescence measurements were performed using a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). Turn on the power to the FilterMax F5. FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices. Intended Use Statement The Synergy H1 is a hybrid multi-mode microplate reader. Add to my manuals. Instrument Setup: 1. MetaMorph NX. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. #E1910) in a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). GFP expression differences. F5 Multi-Mode Microplate Reader. In addition, the FilterMax F5 Reader is capable of measuring nucleic acids directly at 260 nm in absorbance detection mode. Methods for MycoAlert and MycoAlert PLUS Assays are basically the same, except for the concentration range of assay control used, and are detailed below. #F5) Methods Standard curves were generated following the assay protocols provided with each kit. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. Supplier: Molecular Devices. d. The following document is intended to demonstrate setup of this instrument. Unsuccessful migration. Other recommended products. 09 CAD. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell. The multi-mode microplate reader provides flexibility and ease of use for a variety of applications Market Highlights Microplate Readers Market is predicted to project a notable CAGR of 4. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 4 (Molecular Devices, Sunnyvale, CA, USA) at room temperature (fluorimeter settings used were as follows: measurement type, kinetic, 60 cycle, 60-s interval; excitation wavelength, 360 nm;. #6590-0121)The MTT solution was withdrawn, and 200 µl of dimethyl sulphoxide (DMSO) was added to determine the absorbance values, at an absorption curve of λ= 595 nm (FilterMax F5 Multi-Mode Microplate reader, Molecular Probes). How to Get Multi-Day Kinetic Data. 00The microplate reader market was valued at US$ 534. The quantity of colorimetric product was continuously monitored for 120 min. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seEye lysates were developed using a Glucose Colorimetric Assay Kit (Sigma, Louis, MO, USA) and measured by a FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 570 nm. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). FilterMax F5 Multi-Mode Microplate Reader. Turn on the power switch on the back of the instrument. The following document is intended to demonstrate setup of this instrument. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. 0. 2. CloneMatrix. Published Dec 16, 2020 | Updated Dec 16, 2020. 3 Pages. com B. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. 4. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Molecular Devices Multi-Mode Microplate Reader FilterMax F5 $7,995. 6% from 2020 to 2027. d. HERM LB 500 with NaI detector Radio HPLC Detector. Figure 2-23: Bottle holder To install the bottle holder: 1. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solu. Table of Contents. Neurotransmitter Transporter Uptake Assay Kit. Important things to consider when purchasing a multimode microplate reader. After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. Cytokines Assay. 4. A microplate consists of small wells wherein separate reactions take place. 9 mM H 2 O 2 for. 030 fmol Fluorescence Polarization (FP) (Fluorescein 1 nM, SD) 96-well (200 µL) 0. 25. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. All ELISA kits were read using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices; San Jose, CA, USA), according to the kit guidelines. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Statistical analysis was performed using. Maximum haemolysis was obtained using ultrapure distilled water, and untreated erythrocytes were used as a negative control [14]. The microplates were incubated at 37°C for 24 h, and the growth of bacteria was determined by measuring the optical density (OD) at 630 nm using an ELISA microplate reader (FilterMax F5 multi-mode microplate reader, Molecular Devices, Sunnyvale, CA, USA). 7°C (gradient heating design). MetaFluor Software. MetaFluor Software Brochure. Click to Contact Seller. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched value and flexibility for a variety of applications capable of performing: - Absorbance detection in the visible range (340 nm to 650 nm, F3) or UV and visible range (230nm to. Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 666 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. These assays span over a wide wavelength range, generally from 220-900 nm in different detection modes (typically absorbance, fluorescence and luminescence). After incubation, each microplate was analyzed for absorbance reading. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Owner, Zeckhausen. GenePix 4300/4400 Microarray Scanner. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. 9. 4 Pages. Molecular Devices Microplate reader VersaMax $1,495. Multi-mode microplate readers are used in different research fields for the quantification of different biological and chemical assays in a microplate. 1. Method Fluorescence intensity Read Type Endpoint / Kinetic Optics Type Filters Step Label "Linearity Read" Read well C1-F5 Filter Set. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. CloneMatrix. We have 2 Molecular Devices FilterMax F5 manuals available for free PDF download:. Run a variety of luminometric research applications with the Thermo Scientific Luminoskan microplate luminometer, a compact and robust instrument offering versatile plate formats, fast reading speeds and up to three dispensers. SpectraMax Glo Steady-Luc Reporter Assay Kit. This minor release branch supports the B2100 (A109) hardware product until the product's End of Software. 2. Recommended Optics The lysates were incubated in caspase-3 substrate Ac-DEVD-pNA at 37 °C. 3 Pages. The amount of colorimetric product was continuously monitored for 120 min. In addition, the FilterMax F5 Reader is capable of measuring. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Bioprocess Equipment and Technology. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. FilterMax F3 & F5 Microplate Readers. RNA extraction and reverse transcriptase–quantitative PCR. The VICTOR Nivo multimode microplate reader is a high-performance filter-based plate reader equipped with all major detection technologies – Alpha, luminescence, etc. Accurate measurements are ensured by automatic lamp calibration prior to each reading. Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat SYRINGE/VALVE CHAMBER FOR RQF INSTRUMENT quench flow analysis KIN-TEK RAPID QUENCH FLOW INSTRUMENT KIN-TEK RQF-3 VALVE/SYRINGE CHAMBER Biorad Laboratory Real Time PCR System& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:FilterMax F5 Multi-Mode Microplate Readers, 96 to 384 Well or 6 to 1536 Well, Molecular Devices, LLC (CAT#: STEM-LE-0509-LC) Mithras LB 940 Multimode Microplate Reader, 200-1000 nm Spectral Range Absorbance, BERTHOLD TECHNOLOGIES GmbH & Co. Microplate readers. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. Watson. Applications: Absorbance, Cell viability, ELISA, Fluorescence,. 3 Pages. Instrument Configuration: Assay Technology Adapta® & Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET only) (TR-FRET only) HTRF Certification LanthaScreen Certification Key. A standard curve was created by diluting the ATP standards to a range between 2 × 10 −3 and 20 μM ATP. , Sunnyvale, CA, USA) for 30 min and 1 h after dye addition. [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. 4 Pages. Luminoskan luminometric microplate reader. In addition, the instrument features linear and orbital shaking, plus temperature. The supernatant was taken and 0. Fluorescence intensity was determined using a FilterMax F5 multi-mode microplate reader at an excitation wavelength of 485 and an emission wavelength of 530nm. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $ 7,900. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. Gently pull the plastic tab protruding from the microplate chamber door. They provide absorbance, fluorescence, glow luminescence, and other read modes for. 4 Pages. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . ** Some compatible filter sets are available for. MetaXpress. , Shanghai, China) and microplate reader (FilterMax F5 Multi-Mode Microplate Reader, LLC, Molecular Devices, San Jose, CA, United States) at a wavelength of 450 nm. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. . 713579).